Contrast Techniques in Light Microscopy
نویسندگان
چکیده
منابع مشابه
Contrast Enhancement in Light Microscopy
Optical microscopes, which are among the oldest instruments of scientific discovery, continue to be key tools in both biomedical research and routine diagnosis. This remains true despite the development of a wide range of new imaging technologies, many with far greater resolution—ranging from electron microscopes to the multitude of scanning probe systems available today. The overall simplicity...
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Phase contrast microscopy allows the study of highly transparent yet detail-rich specimens by producing intensity contrast from phase objects within the sample. Presented here is a generalized phase contrast illumination schema in which condenser optics are entirely abrogated, yielding a condenser-free yet highly effective method of obtaining phase contrast in transmitted-light microscopy. A ri...
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Confocal laser scanning rnicroscopy (CLSM) was used to examine living organisms within marine biofilms growing on microscope slides and on natural opaque and uneven substrata such as rocks and shells. The results were compared to images of identical fields observed under transmitted llght and phase contrast microscopy and scanning electron microscopy. Confocal microscopy proved superior in sevc...
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ژورنال
عنوان ژورنال: Microscopy Today
سال: 1996
ISSN: 1551-9295,2150-3583
DOI: 10.1017/s1551929500063628